HPRT Antibody (JU03-26)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75528

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JU03-26

Concentration

1 mg/ml

View all HPRT Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human HPRT aa 169-218 / 218. (SwissProt: P00492 Human; SwissProt: P00493 Mouse; SwissProt: P27605 Rat)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HPRT Antibody (JU03-26)

Western Blot: HPRT Antibody (JU03-26) [NBP2-75528]

Western Blot: HPRT Antibody (JU03-26) [NBP2-75528]

Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Western blot analysis of HPRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: MCF-7 cell lysate Lane 2: 293 cell lysate Lane 2: Hela cell lysate Lane 2: A431 cell lysate
Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]

Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528]

Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.
Western Blot: HPRT Antibody (JU03-26) [NBP2-75528]

Western Blot: HPRT Antibody (JU03-26) [NBP2-75528]

Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of HPRT on Hela cell using anti-HPRT antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: A431Lane 3: MCF-7 Lane 4: 293Lane 5: Rat kidney Lane 6: Rat brainLane 7: Mouse testis Lane 8: Mouse colon
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Applications for HPRT Antibody (JU03-26)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000
Application Notes
IP-Assay dependent

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HPRT

HPRT is encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Long Name

Hypoxanthine-Guanine Phosphoribosyltransferase

Alternate Names

HGPRT, HGPRT1, HPRT1

Gene Symbol

HPRT1

Additional HPRT Products

Product Documents for HPRT Antibody (JU03-26)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HPRT Antibody (JU03-26)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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