HPRT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92006

Novus Biologicals, part of Bio-Techne
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NBP1-92006
NBP1-92006-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HPRT Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SDDEPGYDLDLFCIPNHYAEDLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HPRT Antibody

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006]

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006]

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Analysis in human testis and skeletal muscle tissues. Corresponding HPRT RNA-seq data are presented for the same tissues.
Western Blot: HPRT Antibody [NBP1-92006]

Western Blot: HPRT Antibody [NBP1-92006]

Western Blot: HPRT Antibody [NBP1-92006] - Analysis in human cell line HEK 293.
Immunocytochemistry/ Immunofluorescence: HPRT Antibody [NBP1-92006]

Immunocytochemistry/ Immunofluorescence: HPRT Antibody [NBP1-92006]

Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-92006] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
View 4 more images

Applications for HPRT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HPRT

HPRT is encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Long Name

Hypoxanthine-Guanine Phosphoribosyltransferase

Alternate Names

HGPRT, HGPRT1, HPRT1

Entrez Gene IDs

3251 (Human)

Gene Symbol

HPRT1

UniProt

P00492

Additional HPRT Products

Product Documents for HPRT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HPRT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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