HPV16 E1+E4 Antibody (HPV16 E1/E4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07260

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Virus

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HPV16 E1/E4

Concentration

0.2 mg/ml

View all HPV16 E1+E4 Antibodies »

Product Specifications

Immunogen

Recombinant human HPV16 E1+E4 fragment (aa 36-41).

Reactivity Notes

Type 16 of Human Papilloma Virus (HPV-16).

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08319)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for HPV16 E1+E4 Antibody (HPV16 E1/E4)

Immunohistochemistry-Paraffin: HPV16 E1+E4 Antibody (HPV16 E1/E4) [NBP3-07260]

Immunohistochemistry-Paraffin: HPV16 E1+E4 Antibody (HPV16 E1/E4) [NBP3-07260]

Immunohistochemistry-Paraffin: HPV16 E1+E4 Antibody (HPV16 E1/E4) [NBP3-07260] - Formalin-fixed, paraffin-embedded human Cervix stained with HPV16 E1+E4 Mouse Monoclonal Antibody (HPV16 E1/E4).
Immunohistochemistry-Paraffin: HPV16 E1+E4 Antibody (HPV16 E1/E4) [NBP3-07260]

Immunohistochemistry-Paraffin: HPV16 E1+E4 Antibody (HPV16 E1/E4) [NBP3-07260]

Immunohistochemistry-Paraffin: HPV16 E1+E4 Antibody (HPV16 E1/E4) [NBP3-07260] - Formalin-fixed, paraffin-embedded human Cervix stained with HPV16 E1+E4 Mouse Monoclonal Antibody (HPV16 E1/E4).

Applications for HPV16 E1+E4 Antibody (HPV16 E1/E4)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HPV16 E1+E4

HPV16 is an ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans. This antibody is of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.

Alternate Names

ATP dependent helicase E1, E1, E4, HPV 16 ATP dependent helicase E1, HPV 16 E1, HPV 16 E4, HPV 16 replication protein E1, HPV16 ATP dependent helicase E1, HPV16 E1, HPV16 E4, HPV16 replication protein E1, Human papilloma virus type 16, Human papilloma virus type 16 ATP dependent helicase E1, Human papilloma virus type 16 E1, Human papilloma virus type 16 E4, Human papilloma virus type 16 replication protein E1, Human papillomavirus type 16, Human papillomavirus type 16 ATP dependent helicase E1, Human papillomavirus type 16 E1, Human papillomavirus type 16 E4, Human papillomavirus type 16 replication protein E1, Replication protein, Replication protein E1

Additional HPV16 E1+E4 Products

Product Documents for HPV16 E1+E4 Antibody (HPV16 E1/E4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HPV16 E1+E4 Antibody (HPV16 E1/E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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