Key Product Details

Species Reactivity

Virus

Applications

ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TVG 402

Format

BSA Free

Concentration

1 mg/ml

View all HPV16 E1+E4 Antibodies »

Product Specifications

Immunogen

Antigen for hybridoma production was expressed as a beta galactosidase fusion protein using an expression system and was consequently cleaved to release the E1/E4 polypeptide.

Reactivity Notes

Human papilloma virus

Localization

Nuclear

Specificity

The epitope recognized by this antibody survived brief fixation in 5% formaldehyde, but not prolonged fixation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

10 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for HPV16 E1+E4 Antibody (TVG 402) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HPV16 E1+E4

HPV16 is an ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. HPV16, in comparison to HPV types 6 and 11, is more often associated with malignant genital cancers in humans. This antibody is of value in monitoring the progress of HPV infection from benign lesions to invasive cancer.

Alternate Names

ATP dependent helicase E1, E1, E4, HPV 16 ATP dependent helicase E1, HPV 16 E1, HPV 16 E4, HPV 16 replication protein E1, HPV16 ATP dependent helicase E1, HPV16 E1, HPV16 E4, HPV16 replication protein E1, Human papilloma virus type 16, Human papilloma virus type 16 ATP dependent helicase E1, Human papilloma virus type 16 E1, Human papilloma virus type 16 E4, Human papilloma virus type 16 replication protein E1, Human papillomavirus type 16, Human papillomavirus type 16 ATP dependent helicase E1, Human papillomavirus type 16 E1, Human papillomavirus type 16 E4, Human papillomavirus type 16 replication protein E1, Replication protein, Replication protein E1

Additional HPV16 E1+E4 Products

Product Documents for HPV16 E1+E4 Antibody (TVG 402) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HPV16 E1+E4 Antibody (TVG 402) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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