Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all HR6A/UBE2A Antibodies »

Product Specifications

Immunogen

A synthetic peptide which maps to a region between residues 10 and 50 of human ubiquitin-conjugating enzyme E2A using the numbering given in Swiss-Prot entry P49459 (GeneID 7319).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HR6A/UBE2A Antibody

Western Blot: HR6A/UBE2A Antibody [NB100-554]

Western Blot: HR6A/UBE2A Antibody [NB100-554]

Western Blot: HR6A/UBE2A Antibody [NB100-554] - Detection of Human Rad6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad6 antibody NB100-554 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: HR6A/UBE2A Antibody [NB100-554]

Immunohistochemistry: HR6A/UBE2A Antibody [NB100-554]

Immunohistochemistry: HR6A/UBE2A Antibody [NB100-554] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti- Rad6 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: HR6A/UBE2A Antibody [NB100-554]

Western Blot: HR6A/UBE2A Antibody [NB100-554]

Western Blot: HR6A/UBE2A Antibody [NB100-554] - Whole cell lysate (50 ug) from 293T cells.

Applications for HR6A/UBE2A Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Western Blot

1:5000-1:25000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HR6A/UBE2A

The rad6 protein catalyzes the monoubiquitination of H2B. Rad6-directed H2B ubiquitination defines regions of active chromatin. The RAD6 pathway is also central to post-replicative DNA repair in eukaryotic cells.

Long Name

Ubiquitin-conjugating Enzyme E2A

Alternate Names

RAD6A, UBE2A

Entrez Gene IDs

7319 (Human)

Gene Symbol

UBE2A

UniProt

P49459 (Human)

Additional HR6A/UBE2A Products

Product Documents for HR6A/UBE2A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HR6A/UBE2A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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