HR6A/UBE2A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-555
![Western Blot: HR6A/UBE2A Antibody [NB100-555]](https://resources.bio-techne.com/images/products/HR6A-UBE2A-Antibody-Western-Blot-NB100-555-img0008.jpg "Western Blot: HR6A/UBE2A Antibody [NB100-555]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A synthetic peptide which maps to a region between residues 100 and 150 of human ubiquitin-conjugating enzyme E2A using the numbering given in Swiss-Prot entry P49459 (GeneID 7319).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HR6A/UBE2A Antibody
Western Blot: HR6A/UBE2A Antibody [NB100-555]
Western Blot: HR6A/UBE2A Antibody [NB100-555] - Detection of Human Rad6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rad6 antibody NB100-555 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Western Blot: HR6A/UBE2A Antibody [NB100-555]](https://resources.bio-techne.com/images/products/HR6A-UBE2A-Antibody-Western-Blot-NB100-555-img0003.jpg "Western Blot: HR6A/UBE2A Antibody [NB100-555]")
Western Blot: HR6A/UBE2A Antibody [NB100-555]
Western Blot: HR6A/UBE2A Antibody [NB100-555] - Whole cell lysate (50 ug) from 293T cells.Applications for HR6A/UBE2A Antibody
Application
Recommended Usage
Western Blot
1:5000-1:25000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:5,000 - 1:25,000 Immunoprecipitation: not tested Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HR6A/UBE2A
Long Name
Ubiquitin-conjugating Enzyme E2A
Alternate Names
RAD6A, UBE2A
Entrez Gene IDs
7319 (Human)
Gene Symbol
UBE2A
UniProt
Additional HR6A/UBE2A Products
Product Documents for HR6A/UBE2A Antibody
Product Specific Notices for HR6A/UBE2A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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