HR6B/UBE2B Antibody (1F11)
Novus Biologicals, part of Bio-Techne | Catalog # H00007320-M13
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1F11
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
UBE2B (AAH08470.1, 1 a.a. ~ 152 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS
Specificity
UBE2B - ubiquitin-conjugating enzyme E2B (RAD6 homolog) (1F11)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for HR6B/UBE2B Antibody (1F11)
![Western Blot: HR6B/UBE2B Antibody (1F11) [H00007320-M13]](https://resources.bio-techne.com/images/products/HR6B-UBE2B-Antibody-1F11-Western-Blot-H00007320-M13-img0002.jpg "Western Blot: HR6B/UBE2B Antibody (1F11) [H00007320-M13]")
Western Blot: HR6B/UBE2B Antibody (1F11) [H00007320-M13]
Western Blot: HR6B/UBE2B Antibody (1F11) [H00007320-M13] - Analysis of UBE2B expression in transfected 293T cell line by UBE2B monoclonal antibody (M13), clone 1F11. Lane 1: UBE2B transfected lysatE (17.3 KDa). Lane 2: Non-transfected lysate.
Sandwich ELISA: HR6B/UBE2B Antibody (1F11) [H00007320-M13] - Detection limit for recombinant GST tagged UBE2B is 1 ng/ml as a capture antibody.
Applications for HR6B/UBE2B Antibody (1F11)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HR6B/UBE2B
Long Name
Ubiquitin-conjugating Enzyme E2B
Alternate Names
E2-17k, UBE2B
Entrez Gene IDs
7320 (Human)
Gene Symbol
UBE2B
UniProt
Additional HR6B/UBE2B Products
Product Documents for HR6B/UBE2B Antibody (1F11)
Product Specific Notices for HR6B/UBE2B Antibody (1F11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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