HR6B/UBE2B Antibody (2Z3R2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15271
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2Z3R2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 53-152 of human HR6B/UBE2B (P63146). FKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HR6B/UBE2B Antibody (2Z3R2)
Western Blot: HR6B/UBE2B Antibody (2Z3R2) [NBP3-15271]
Western Blot: HR6B/UBE2B Antibody (2Z3R2) [NBP3-15271] - Western blot analysis of extracts of Rat testis, using HR6B/UBE2B antibody (NBP3-15271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunohistochemistry-Paraffin: HR6B/UBE2B Antibody (2Z3R2) [NBP3-15271]
Immunohistochemistry-Paraffin: HR6B/UBE2B Antibody (2Z3R2) [NBP3-15271] - Immunohistochemistry of paraffin-embedded rat brain using HR6B/UBE2B Rabbit mAb (NBP3-15271) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.Western Blot: HR6B/UBE2B Antibody (2Z3R2) [NBP3-15271]
Western Blot: HR6B/UBE2B Antibody (2Z3R2) [NBP3-15271] - Western blot analysis of extracts of various cell lines, using HR6B/UBE2B antibody (NBP3-15271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.Applications for HR6B/UBE2B Antibody (2Z3R2)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HR6B/UBE2B
Long Name
Ubiquitin-conjugating Enzyme E2B
Alternate Names
E2-17k, UBE2B
Gene Symbol
UBE2B
Additional HR6B/UBE2B Products
Product Documents for HR6B/UBE2B Antibody (2Z3R2)
Product Specific Notices for HR6B/UBE2B Antibody (2Z3R2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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