HRAS Antibody (4Q7M5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15818
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4Q7M5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 90-189 of human HRAS (P01112). FEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HRAS Antibody (4Q7M5)
![Western Blot: HRAS Antibody (4Q7M5) [NBP3-15818]](https://resources.bio-techne.com/images/products/HRAS-Antibody-4Q7M5-Western-Blot-NBP3-15818-img0002.jpg "Western Blot: HRAS Antibody (4Q7M5) [NBP3-15818]")
Western Blot: HRAS Antibody (4Q7M5) [NBP3-15818]
Western Blot: HRAS Antibody (4Q7M5) [NBP3-15818] - Western blot analysis of extracts of various cell lines, using HRAS antibody (NBP3-15818) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunohistochemistry-Paraffin: HRAS Antibody (4Q7M5) [NBP3-15818]](https://resources.bio-techne.com/images/products/HRAS-Antibody-4Q7M5-Immunohistochemistry-Paraffin-NBP3-15818-img0001.jpg "Immunohistochemistry-Paraffin: HRAS Antibody (4Q7M5) [NBP3-15818]")
Immunohistochemistry-Paraffin: HRAS Antibody (4Q7M5) [NBP3-15818]
Immunohistochemistry-Paraffin: HRAS Antibody (4Q7M5) [NBP3-15818] - Immunohistochemistry of paraffin-embedded human breast cancer using HRAS antibody (NBP3-15818) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for HRAS Antibody (4Q7M5)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HRAS
Alternate Names
C-BAS/HAS;C-HA-RAS1;C-H-RAS;CTLO;GTPase HRas;HAMSV;HRAS1;H-RASIDX;p21ras;RASH1
Gene Symbol
HRAS
Additional HRAS Products
Product Documents for HRAS Antibody (4Q7M5)
Product Specific Notices for HRAS Antibody (4Q7M5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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