HRAS Antibody (OTI1D9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45745
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]](https://resources.bio-techne.com/images/products/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0007.jpg "Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]")
![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]](https://resources.bio-techne.com/images/products/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0006.jpg "Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Monkey
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1D9
Concentration
1 mg/ml
Product Summary for HRAS Antibody (OTI1D9)
Immunogen
Full length human recombinant protein of human HRAS(NP_005334) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HRAS Antibody (OTI1D9)
Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]
Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]](https://resources.bio-techne.com/images/products/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0004.jpg "Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]")
Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]
Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]](https://resources.bio-techne.com/images/products/HRAS-Antibody-OTI1D9-Western-Blot-NBP2-45745-img0005.jpg "Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]")
Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745]
Western Blot: HRAS Antibody (OTI1D9) [NBP2-45745] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY vH RAS.![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]](https://resources.bio-techne.com/images/products/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0002.jpg "Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]")
![Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]](https://resources.bio-techne.com/images/products/HRAS-Antibody-OTI1D9-Immunohistochemistry-NBP2-45745-img0003.jpg "Immunohistochemistry: HRAS Antibody (OTI1D9) [NBP2-45745]")
![Immunofluorescence: HRAS Antibody (OTI1D9) [NBP2-45745]](https://resources.bio-techne.com/images/products/HRAS-Antibody-OTI1D9-Immunofluorescence-NBP2-45745-img0001.jpg "Immunofluorescence: HRAS Antibody (OTI1D9) [NBP2-45745]")
Applications for HRAS Antibody (OTI1D9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:200-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HRAS
Alternate Names
C-BAS/HAS;C-HA-RAS1;C-H-RAS;CTLO;GTPase HRas;HAMSV;HRAS1;H-RASIDX;p21ras;RASH1
Gene Symbol
HRAS
Additional HRAS Products
Product Documents for HRAS Antibody (OTI1D9)
Product Specific Notices for HRAS Antibody (OTI1D9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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