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HRAS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42864

Novus Biologicals, part of Bio-Techne
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NBP2-42864

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Chicken (100%), Rhesus Macaque (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HRAS. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (100%).

Specificity

This antibody is specific for H-Ras protein, and it does not cross react with human N-Ras or K-Ras protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HRAS Antibody

Western Blot: HRAS Antibody [NBP2-42864]

Western Blot: HRAS Antibody [NBP2-42864]

Western Blot: HRAS Antibody [NBP2-42864] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: HRAS Antibody [NBP2-42864]

Immunocytochemistry/ Immunofluorescence: HRAS Antibody [NBP2-42864]

Immunocytochemistry/Immunofluorescence: HRAS Antibody [NBP2-42864] - Analysis of paraformaldehyde-fixed HeLa, using H-Ras antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: HRAS Antibody [NBP2-42864]

Immunohistochemistry-Paraffin: HRAS Antibody [NBP2-42864]

Immunohistochemistry-Paraffin: HRAS Antibody [NBP2-42864] - Mouse intestine. H-RAS antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for HRAS Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HRAS

Alternate Names

C-BAS/HAS;C-HA-RAS1;C-H-RAS;CTLO;GTPase HRas;HAMSV;HRAS1;H-RASIDX;p21ras;RASH1

Gene Symbol

HRAS

Additional HRAS Products

Product Documents for HRAS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HRAS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.