HSCB Antibody (OTI3E1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72415

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3E1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HSCB Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSCB(NP_741999) produced in HEK293T cell

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSCB Antibody (OTI3E1) - Azide and BSA Free

Western Blot: HSCB Antibody (OTI3E1)Azide and BSA Free [NBP2-72415]

Western Blot: HSCB Antibody (OTI3E1)Azide and BSA Free [NBP2-72415]

Western Blot: HSCB Antibody (OTI3E1) - Azide and BSA Free [NBP2-72415] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSCB.
Immunohistochemistry: HSCB Antibody (OTI3E1) - Azide and BSA Free [NBP2-72415]

Immunohistochemistry: HSCB Antibody (OTI3E1) - Azide and BSA Free [NBP2-72415]

Immunohistochemistry: HSCB Antibody (OTI3E1) - Azide and BSA Free [NBP2-72415] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: HSCB Antibody (OTI3E1)Azide and BSA Free [NBP2-72415]

Western Blot: HSCB Antibody (OTI3E1)Azide and BSA Free [NBP2-72415]

Western Blot: HSCB Antibody (OTI3E1) - Azide and BSA Free [NBP2-72415] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Applications for HSCB Antibody (OTI3E1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:400-4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSCB

HSCB may act as a co-chaperone in iron-sulfur cluster assembly in mitochondria

Alternate Names

DnaJ homolog subfamily C member 20, DNAJC20DnaJ (Hsp40) homolog, subfamily C, member 20, Hsc20, HSC20dJ366L4.2, HscB iron-sulfur cluster co-chaperone homolog (E. coli), iron-sulfur cluster co-chaperone protein HscB, mitochondrial, JAC1, J-type co-chaperone HSC20, MGC2637, MGC74462

Gene Symbol

HSCB

Additional HSCB Products

Product Documents for HSCB Antibody (OTI3E1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSCB Antibody (OTI3E1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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