HSD3B1 Antibody (10G2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26218
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10G2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HSD3B1 Antibody (10G2)
Immunogen
A synthesized peptide derived from Human HSD3B1 [UniProt P14060]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HSD3B1 Antibody (10G2)
Immunohistochemistry: HSD3B1 Antibody (10G2) [NBP3-26218] -
Immunohistochemistry: HSD3B1 Antibody (10G2) [NBP3-26218] - Image of HSD3B1 Antibody (10G2) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.50% DAB.
Immunohistochemistry: HSD3B1 Antibody (10G2) [NBP3-26218] -
Immunohistochemistry: HSD3B1 Antibody (10G2) [NBP3-26218] - Image of HSD3B1 Antibody (10G2) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.50% DAB.
Immunocytochemistry/Immunofluorescence: HSD3B1 Antibody (10G2) [NBP3-26218] -
Immunocytochemistry/Immunofluorescence: HSD3B1 Antibody (10G2) [NBP3-26218] - Staining of Hela with HSD3B1 Antibody (10G2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 514-conjugated Goat Anti-Rabbit IgG (H+L).Applications for HSD3B1 Antibody (10G2)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: HSD3B1
HSD3B1 is required for the production of progesterone by the placenta, so it is predicted that mutations in this gene may cause infertility in women. HSD3B1 antibodies are useful tools for researchers studying hormone regulation and reproductive biology.
Alternate Names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1, 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I, 3BETAHSD, 3-beta-HSD I, 3-beta-hydroxy-Delta(5)-steroid dehydrogenase, 3BH, delta-5-3-ketosteroid isomerase, EC 1.1.1, HSD3B, HSDB3, HSDB3A, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1, hydroxy-delta-5-steroid dehydrogenase, 3 beta-and steroid delta-isomerase 1, I, progesterone reductase, SDR11E1, short chain dehydrogenase/reductase family 11E, member 1,3-beta-hydroxy-5-ene steroid dehydrogenase, steroid Delta-isomerase, Trophoblast antigen FDO161G
Gene Symbol
HSD3B1
Additional HSD3B1 Products
Product Documents for HSD3B1 Antibody (10G2)
Product Specific Notices for HSD3B1 Antibody (10G2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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