HSF1 Antibody (S07-0G0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14895

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-14895-100ul
NBP3-14895-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-0G0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSF1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human HSF1 (Uniprot#: Q00613)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSF1 Antibody (S07-0G0)

Western Blot: HSF1 Antibody (S07-0G0) [NBP3-14895]

Western Blot: HSF1 Antibody (S07-0G0) [NBP3-14895]

Western Blot: HSF1 Antibody (S07-0G0) [NBP3-14895] - Western blot detection of HSF1 in Hela, Jurkat cell lysates using HSF1 Rabbit mAb (1:500 diluted). Predicted band size: 57kDa. Observed band size: 80kDa.
Immunohistochemistry-Paraffin: HSF1 Antibody (S07-0G0) [NBP3-14895]

Immunohistochemistry-Paraffin: HSF1 Antibody (S07-0G0) [NBP3-14895]

Immunohistochemistry-Paraffin: HSF1 Antibody (S07-0G0) [NBP3-14895] - Immunohistochemistry of HSF1 in paraffin-embedded Human breast cancer tissue using HSF1 Rabbit mAb at dilution 1/50

Applications for HSF1 Antibody (S07-0G0)

Application
Recommended Usage

Immunohistochemistry

1/20

Western Blot

1/2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSF1

Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. HSF1-symplekin complexes are detected only after stress treatment, and these two proteins co-localize in punctate nuclear structures in stressed cells (2). A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. It has been concluded that Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1 (3).

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Gene Symbol

HSF1

Additional HSF1 Products

Product Documents for HSF1 Antibody (S07-0G0)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSF1 Antibody (S07-0G0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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