HSF2 Antibody (OTI3E1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70948

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3E1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HSF2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 74-324 of human HSF2(NP_004497) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSF2 Antibody (OTI3E1) - Azide and BSA Free

Western Blot: HSF2 Antibody (OTI3E1)Azide and BSA Free [NBP2-70948]

Western Blot: HSF2 Antibody (OTI3E1)Azide and BSA Free [NBP2-70948]

Western Blot: HSF2 Antibody (OTI3E1) - Azide and BSA Free [NBP2-70948] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSF2.
Immunohistochemistry: HSF2 Antibody (OTI3E1) - Azide and BSA Free [NBP2-70948]

Immunohistochemistry: HSF2 Antibody (OTI3E1) - Azide and BSA Free [NBP2-70948]

Immunohistochemistry: HSF2 Antibody (OTI3E1) - Azide and BSA Free [NBP2-70948] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: HSF2 Antibody (OTI3E1) - Azide and BSA Free [NBP2-70948]

Immunohistochemistry: HSF2 Antibody (OTI3E1) - Azide and BSA Free [NBP2-70948]

Immunohistochemistry: HSF2 Antibody (OTI3E1) - Azide and BSA Free [NBP2-70948] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for HSF2 Antibody (OTI3E1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSF2

Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up-regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. HSF2 exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form. Various experiments have suggested that HSF2 may play roles in differentiation and development.

Long Name

Heat Shock Transcription Factor 2

Alternate Names

HSTF2

Gene Symbol

HSF2

Additional HSF2 Products

Product Documents for HSF2 Antibody (OTI3E1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSF2 Antibody (OTI3E1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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