HSP20/HSPB6 Antibody (C9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-41093

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # C9

Concentration

1 mg/ml

View all HSP20/HSPB6 Antibodies »

Product Specifications

Immunogen

Immunized with recombinant Rat HSP20/HSPB6 (Uniprot#: P97541; Met1-Lys162 (Uniprot#: P97541)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for HSP20/HSPB6 Antibody (C9)

HSP20/HSPB6 Antibody (C9)

Immunohistochemistry-Paraffin: HSP20/HSPB6 Antibody (C9) [NBP3-41093] -

Immunohistochemistry-Paraffin: HSP20/HSPB6 Antibody (C9) [NBP3-41093] - DAB staining on IHC-P; Sample: Rat Cardiac Muscle Tissue; Primary Ab: 20ug/ml NBP3-41093; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody;
HSP20/HSPB6 Antibody (C9)

Western Blot: HSP20/HSPB6 Antibody (C9) [NBP3-41093] -

Western Blot: HSP20/HSPB6 Antibody (C9) [NBP3-41093] - Sample: Recombinant HSP20/HSPB6, Rat.
HSP20/HSPB6 Antibody (C9)

Western Blot: HSP20/HSPB6 Antibody (C9) [NBP3-41093] -

Western Blot: HSP20/HSPB6 Antibody (C9) [NBP3-41093] - Sample: Lane1: Rat Heart lysate; Lane1: Rat Skeletal muscle lysate Primary Ab: 1ug/ml NBP3-41093; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody

Applications for HSP20/HSPB6 Antibody (C9)

Application
Recommended Usage

Immunohistochemistry

5-20ug/mL

Western Blot

0.5-2ug/mL

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.01M PBS, pH7.4, 50% glycerol

Preservative

0.05% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP20/HSPB6

Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and may form different hetercomplexes with these proteins. The specific physiological function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions, Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions, it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. The Hsp20 was demonstrated to constitute up to 1.3% of the total cellular protein in vertebrate tissues, especially in muscle, and its expression is related to muscle contraction, specifically in slow-twitch muscle. Hsp20 may form different heterocomplexes with other Hsp's, such as alpha-crystalline and Hsp25. Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments. In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction.

Long Name

Heat Shock Protein 20

Alternate Names

HSPB6

Gene Symbol

HSPB6

Additional HSP20/HSPB6 Products

Product Documents for HSP20/HSPB6 Antibody (C9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSP20/HSPB6 Antibody (C9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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