Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 2079A

Format

Azide and BSA Free

Concentration

1.0 mg/ml

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Product Specifications

Immunogen

Phosphopeptide containing the human HSP27 S78 site
Accession # P04792

Modification

p Ser78

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP27 [p Ser78] Antibody (2079A) - Azide and BSA Free

Western Blot: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Detection of Human HSP27 [p Ser78] by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or UV treated (+). PVDF membrane was probed with Rabbit Anti-Human HSP27 [p Ser78] Monoclonal Antibody (Catalog # MAB9355) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. A specific band was detected for HSP27 [p Ser78 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions
Flow Cytometry: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - An intracellular stain was performed on MCF7 cells with HSP27 [p Ser78] Antibody MAB9355 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).
Simple Western: HSP27 [p Ser78] Antibody (2079A) [MAB9355] - Lane vies shows a specific band for HSP27 using 0.2 mg/mL MCF-7 -/+ UV cell lysates and antibody at 5 ug/mL. Electropherogram image of corresponding Simple Western lanes view at WES molecular weight of 32 kDa. Image reported by internal validation.

Applications for HSP27 [p Ser78] Antibody (2079A) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for HSP27 [p Ser78] Antibody (2079A) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP27 [p Ser78] Antibody (2079A) - Azide and BSA Free

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This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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