HSP27 [p Ser78] Antibody (JBW502)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29821

Novus Biologicals, part of Bio-Techne
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NBP2-29821
NBP2-29821-0.025mg
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # JBW502

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSP27 Antibodies »

Product Specifications

Immunogen

peptide corresponding to amino acids 73-86 of human HSP27 with a C-terminus cysteine added for conjugation.

Reactivity Notes

Human

Modification

p Ser78

Specificity

Detects phosphorylated HSP27 (Ser78).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Relevant Entrez Gene Numbers: NM_001540

Scientific Data Images for HSP27 [p Ser78] Antibody (JBW502)

Western Blot: HSP27 [p Ser78] Antibody (JBW502) [NBP2-29821]

Western Blot: HSP27 [p Ser78] Antibody (JBW502) [NBP2-29821]

Western Blot: Hsp27 Antibody (JBW502) [NBP2-29821] - Untreated (lane 1) and heat shock and arsenite treated (lane 2) MCF-7 cell lysate was probed with 0.05ug/ml of anti-phospho-HSP27 (Ser 78). Arrow indicates phosphorylated HSP27.

Applications for HSP27 [p Ser78] Antibody (JBW502)

Application
Recommended Usage

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.1M Tris-Glycine (phH 7.4), 0.15M NaCl and 30% Glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

UniProt

P04792

Additional HSP27 Products

Product Documents for HSP27 [p Ser78] Antibody (JBW502)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP27 [p Ser78] Antibody (JBW502)

Relevant Entrez Gene Numbers: NM_001540

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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