HSP27 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-75477

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Bovine (95%), Canine (93%), Hamster (95%), Porcine (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all HSP27 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of the human Hsp27 protein (within residues 80-150). [Swiss-Prot# P04792]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (80%).

Localization

Cytoplasm and nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP27 Antibody - BSA Free

Knockout Validated: HSP27 Antibody - BSA Free [NBP1-75477]

Knockout Validated: HSP27 Antibody - BSA Free [NBP1-75477]

Knockout Validated: HSP27 Antibody [NBP1-75477] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human HSP27 Polyclonal Antibody (Catalog # NBP1-75477) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (catalog number HAF008). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: HSP27 AntibodyBSA Free [NBP1-75477]

Western Blot: HSP27 AntibodyBSA Free [NBP1-75477]

Western Blot: HSP27 Antibody [NBP1-75477] - Analysis of Jurkat cell lysate (30ug) using anti-Hsp27 antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: HSP27 Antibody - BSA Free [NBP1-75477]

Immunocytochemistry/ Immunofluorescence: HSP27 Antibody - BSA Free [NBP1-75477]

Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NBP1-75477] - Hsp27 Antibody [NBP1-75477] - HSP27 antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
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Applications for HSP27 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

2 ug/ml
Application Notes
This HSP27 antibody is useful for ICC/IF, IHC-P, and Western blot where a band is seen ~27 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 3 using NBP1-75477 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP27

Heat shock protein 27 is a molecular chaperone involved in protection of cells during cellular stress events. The activity of HSP27 protects from apoptotic events caused by heat shock and other distress. Immunohistochemical detection of HSP27 has been suggested as a qualitative biomarker for colorectal cancer. It is also highly expressed in lung cancer, cerebral inflammation, ischemia-reperfusion injury, and glaucoma. Interestingly, HSP27 overexpression can counteract the activity of multi-drug resistance proteins in breast cancer. Polyphenols downregulate the expression of HSP27, as well as many antioxidant enzymes, resulting in susceptibility to stress and damage.

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Entrez Gene IDs

3315 (Human); 15507 (Mouse)

Gene Symbol

HSPB1

UniProt

P04792, P14602 (Mouse)

Additional HSP27 Products

Product Documents for HSP27 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP27 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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