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Hsp47 Antibody (10B2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15163

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15163

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10B2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Hsp47 Antibody (10B2)

Immunogen

A synthesized peptide derived from human Hsp47. UniProt ID (P50454).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hsp47 Antibody (10B2)

Immunohistochemistry: Hsp47 Antibody (10B2) [NBP3-15163]

Immunohistochemistry: Hsp47 Antibody (10B2) [NBP3-15163]

Immunohistochemistry: Hsp47 Antibody (10B2) [NBP3-15163] - IHC image of Hsp47 antibody diluted at 1:100 and staining in paraffin-embedded human placenta tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Hsp47 Antibody (10B2)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: HSP47

Hsp47 encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.

Long Name

Heat Shock Protein 47

Alternate Names

AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1

Gene Symbol

SERPINH1

Additional HSP47 Products

Product Documents for Hsp47 Antibody (10B2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hsp47 Antibody (10B2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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