Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, C. elegans, Canine, Chicken, Drosophila, Fish, Guinea Pig, Hamster, Monkey, Plant, Rabbit, Sheep, Xenopus

Cited:

Human, Mouse

Applications

Validated:

Electron Microscopy, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow (Cell Surface), Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # N27F3-4

Concentration

1 mg/ml

View all HSP70/HSPA1A Antibodies »

Product Specifications

Immunogen

Recombinant HSP70/HSC70

Localization

Cytoplasm

Specificity

Detects approx 72 (HSP) and approx 73kDa (HSC).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HSP70/HSPA1A Antibody (N27F3-4)

Western Blot: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425]

Western Blot: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425]

Western Blot: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425] - analysis of Human Cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunohistochemistry: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425]

Immunohistochemistry: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425]

Immunohistochemistry: HSP70/HSPA1A Antibody (N27F3-4) [NB110-96425] - Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone N27F3-4 (NB110-96425). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (NB110-96425) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Applications for HSP70/HSPA1A Antibody (N27F3-4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of HSP70/HSC70 Antibody was sufficient for detection of HSP70/HSC70 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eucaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eucaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Entrez Gene IDs

24472 (Rat)

Gene Symbol

HSPA1B

UniProt

P08107

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody (N27F3-4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP70/HSPA1A Antibody (N27F3-4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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