HSP70/HSPA1A Antibody (W27)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26422

Novus Biologicals, part of Bio-Techne
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NBP2-26422
NBP2-26422-0.05ml
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # W27

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSP70/HSPA1A Antibodies »

Product Specifications

Immunogen

BALB/C mice were injected with purified HSP70 protein.

Reactivity Notes

Human

Localization

Cytoplasmic, nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for HSP70/HSPA1A Antibody (W27)

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (W27) [NBP2-26422]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (W27) [NBP2-26422]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (W27) [NBP2-26422] - Formalin fixed paraffin embedded human breast carcinoma stained with HSP70/HSPA1A antibody (NBP2-26422).

Applications for HSP70/HSPA1A Antibody (W27)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:1000

Immunohistochemistry

1:25-1:100

Immunohistochemistry-Paraffin

1:25-1:100
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HSP70/HSPA1A

The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed proteins. All of the Hsp70 proteins have three major functional domains. An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate), a substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues and a C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. Eukaryotic organisms express several slightly different Hsp70 proteins. Hsc70 or Hsp73; a constitutively expressed chaperone protein, Hsp70 or Hsp72; a stress-induced protein, BiP or Grp78; a protein localized to the endoplasmic reticulum mtHsp70 or Grp75; the mitochondrial Hsp70. Heat shock protein 72 (Hsp72) is the major inducible, 70kDa heat shock protein. It plays a role in many cellular activities including protein synthesis, folding, and translocation into organelles as well as the assembly of multiprotein complexes. Hsc70 typically makes up one to three percent of total cellular protein. The genes are located in the major histocompatibility complex class III region.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody (W27)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP70/HSPA1A Antibody (W27)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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