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HSP70/HSPA1A Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77456CL1

Novus Biologicals, part of Bio-Techne
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NBP1-77456CL1

Key Product Details

Species Reactivity

Human, Rat

Applications

Block/Neutralize, Electron Microscopy, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide made to a C-terminus portion of the human Hsp70 protein (between residues 600-641) [UniProt # P08107]

Reactivity Notes

Use in Human reported in scientific literature (PMID:33760187).

Localization

Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for HSP70/HSPA1A Antibody [CoraFluor™ 1]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Electron Microscopy

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: HSP70/HSPA1A

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSP70/HSPA1A Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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