HSP70/HSPA1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46806

Novus Biologicals, part of Bio-Techne
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NBP2-46806
NBP2-46806-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HSP70/HSPA1A Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ACERAKRTLSSSTQASLEIDSL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP70/HSPA1A Antibody

Western Blot: HSP70/HSPA1A Antibody [NBP2-46806]

Western Blot: HSP70/HSPA1A Antibody [NBP2-46806]

Western Blot: HSP70/HSPA1A Antibody [NBP2-46806] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody [NBP2-46806]

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody [NBP2-46806]

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP2-46806] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP2-46806] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
View 3 more images

Applications for HSP70/HSPA1A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

UniProt

E7EP94 (Human)

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP70/HSPA1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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