HSP70/HSPA1A [p Ser153] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60773

Novus Biologicals, part of Bio-Techne
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NBP2-60773
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSP70/HSPA1A Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr153 conjugated to KLH. Accession # P0DMV8

Modification

p Ser153

Specificity

Specific for the ~70 kDa HSP70/HSPA1A phosphorylated at Ser153. Immunolabeling of HSP70/HSPA1A is completely eliminated with lambda-phosphatase treatment. Additional bands detected are thought to be other members of the Hsp70 family.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP70/HSPA1A [p Ser153] Antibody

Western Blot: HSP70/HSPA1A [p Ser153] Antibody [NBP2-60773]

Western Blot: HSP70/HSPA1A [p Ser153] Antibody [NBP2-60773]

Western Blot: HSP70/HSPA1A [p Ser153] Antibody [NBP2-60773] - Western blot of T47D cell lysate treated with EGF (1nM) for 60minutes showing specific labeling of the ~70 kDa Hsp70 in thefirst lane (-). Phosphospecificity is shown in the second lane(+) where immunolabeling is eliminated or significantlydecreased with lambda phosphatase (Lambda-Ptase, 1200 units for30 minutes).

Applications for HSP70/HSPA1A [p Ser153] Antibody

Application
Recommended Usage

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A [p Ser153] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP70/HSPA1A [p Ser153] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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