HSP90 alpha Antibody

Western Blot: HSP90 alpha Antibody [NB120-2928]

Western Blot: HSP90 alpha Antibody [NB120-2928] - Figure 1 shows a Western blot of mouse HSP84 and HSP86 using NB120-2928 and NB120-2927 respectively.

Western Blot: HSP90 alpha Antibody [NB120-2928]

Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of epidermoid carcinoma (A431) cells using Heat Shock Protein 86 Polyclonal Antibody. Decreasing amounts of A431 whole cell lysates were probed with PA3-013 at a dilution of 1:2000 followed by an anti-rabbit IgG secondary antibody and SuperSignal West Pico Chemiluminescent Substrate. Lanes 1 and 2 are control samples of purified, recombinant Hsp86 (rHsp86) and Hsp84 (rHsp84), respectively. Data courtesy of the Innovators Program.

Western Blot: HSP90 alpha Antibody [NB120-2928]

Western Blot: HSP90 alpha Antibody [NB120-2928] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP86 polyclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-rabbit IgG HRP secondary antibody at a dilution of 1:20,000 for at least 1 hour.

Western Blot: HSP90 alpha Antibody [NB120-2928]

Western Blot: HSP90 alpha Antibody [NB120-2928] - Analysis of 2-fold serial dilutions of HeLa cell lysate, starting at 10 ug, per well.

Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928]

Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.

Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928]

Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.

Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928]

Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Heat-induced epitope retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 mins at 95C. Following antigen retrieval, tissues blocked in 3% BSA in PBST for 30 mins at RT & then probed with Heat Shock Protein 86 (Hsp86) polyclonal antibody at 1:100 for 1 hr in a humidified chamber (right panel). As a -ve control, primary antibody eliminated from the staining procedure (left panel). Tissues washed with PBS/0.025% Tween-20 & endogenous peroxidase activity quenched with Peroxidase Suppressor for 30 mins at RT. Detection performed using an HRP-conjugated goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:250 followed by colorimetric detection using Metal Enhanced DAB Substrate Kit. Tissues were counterstained with hematoxylin & prepped for mouting. Images were taken on a Zeiss Axiovision microscope at 40X magnification (x1.6 Optovar).

Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928]

Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.

Immunohistochemistry: HSP90 alpha Antibody [NB120-2928]

Immunohistochemistry: HSP90 alpha Antibody [NB120-2928] - HSP90 alpha is upregulated in epithelial cells lining liver cysts.(A, B) Representative hematoxylin stained liver sections with immunohistochemical detection of HSP90 alpha (brown) from three (A) wild type (wt) and (B) Pkd1-/- mice. bd = bile duct, pv = portal vein, c = cyst. Scale bars = 100 um; magnification, 20x. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0114403) licensed under a CC-BY license.

Immunohistochemistry: HSP90 alpha Antibody [NB120-2928]

Immunohistochemistry: HSP90 alpha Antibody [NB120-2928] - HSP90 alpha is upregulated in epithelial cells lining liver cysts.(A, B) Representative hematoxylin stained liver sections with immunohistochemical detection of HSP90 alpha (brown) from three (A) wild type (wt) and (B) Pkd1-/- mice. bd = bile duct, pv = portal vein, c = cyst. Scale bars = 100 um; magnification, 20x. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0114403) licensed under a CC-BY license.

Immunoprecipitation: HSP90 alpha Antibody [NB120-2928]

Immunoprecipitation: HSP90 alpha Antibody [NB120-2928] - Analysis of Heat Shock Protein (HSP86) was performed on HeLa cells. Antigen-antibody complexes formed by incubating 500ug whole cell lysate with 2ug of HSP86 polyclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP86 polyclonal antibody at a dilution of 1:1000 overnight rotating at 4C. The membrane was washed in TBST, and probed with Clean-Blot IP detection Reagent at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.

Immunohistochemistry-Paraffin: HSP90 alpha Antibody [NB120-2928] -

Signaling consequences of STA-8666 treatment.A.-F. Left, Representative images and right, quantification of IHC or immunofluorescence for Ki-67 (A), cleaved caspase 3 (B), pS824-KAP1 (C), pT202/Y204-ERK1/2 (D), vimentin (light red) and DAPI (blue) (E) and HSP90 (F). Magnification: 20x. Scale bars: 75 μm, except for vimentin, where scale bar is 40 μm.