HSP90 Antibody (10D6)

Western Blot: HSP90 Antibody (10D6) [NBP3-15144]

Western Blot: HSP90 Antibody (10D6) [NBP3-15144] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue. All lanes: HSP90 antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 KDa. Observed band size: 90 KDa.

Flow Cytometry: HSP90 Antibody (10D6) [NBP3-15144]

Flow Cytometry: HSP90 Antibody (10D6) [NBP3-15144] - Overlay histogram showing Jurkat cells stained with HSP90 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Immunoprecipitation: HSP90 Antibody (10D6) [NBP3-15144]

Immunoprecipitation: HSP90 Antibody (10D6) [NBP3-15144] - Immunoprecipitating HSP90 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of HSP90 antibody in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: HSP90 antibody (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).