HSP90 Antibody (2D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97450

Novus Biologicals, part of Bio-Techne
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NBP1-97450-0.025ml
NBP1-97450-0.05ml
NBP1-97450-0.2ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Fish, Fungi, Guinea Pig, Hamster, Primate, Rabbit, Sheep

Cited:

Human, Bovine

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgM Clone # 2D12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSP90 Antibodies »

Product Specifications

Immunogen

Native mouse Hsp90.

Reactivity Notes

water mold (100%).

Clonality

Monoclonal

Host

Rat

Isotype

IgM

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP90 Antibody (2D12)

Western Blot: HSP90 Antibody (2D12) [NBP1-97450]

Western Blot: HSP90 Antibody (2D12) [NBP1-97450]

Western Blot: HSP90 Antibody (2D12) [NBP1-97450] - Analysis of HSP90 mAb (2D12). Lane 1: MW marker, Lane 2: HSP90 (human) (native), Lane 3: HeLa HS, (cell lysate) Lane4: Vero Heat Shock Cell Lysate.

Applications for HSP90 Antibody (2D12)

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP90

The Hsp90 family of heat shock proteins represents one of the most abundantly expressed and highly conserved families of cellular chaperones whose expression can be upregulated under conditions of cellular stress, and includes cytoplasmic (Hsp90-alpha/beta), ER (grp94), and mitochondrial (TRAP1) localized members. Structurally, Hsp90 is characterized by an N-terminal ATP-binding domain, a medial substrate-binding domain, and a C-terminal dimerization motif. Hsp90 dimers function in cooperation with cochaperones (e.g. Hsp40, Hsp70, Hop, p23) to stabilize a multitude of client protein substrates, including steroid hormone receptors, protein kinases, and transcription factors. The essential binding and hydrolysis of ATP by Hsp90 is inhibited by ansamycin drugs (e.g. geldanamycin, 17-AAG) which occupy the N-terminal Hsp90 nucleotide-binding pocket. Many Hsp90 client proteins such as erbB2/Her-2, c-raf, bcr-abl, p53, and hTERT, are members of well characterized oncogenic pathways, making Hsp90 inhibitors useful anticancer agents.

Long Name

Heat Shock Protein 90

Alternate Names

FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein HSP 90-alpha, Hsp89, Hsp90, HSP90AHSP86, HSP90N, HSPC1HSP 86, HSPCAHSP89A, HSPCAL1, HSPCAL4, HSPN, LAP2, Renal carcinoma antigen NY-REN-38

Gene Symbol

HSP90AA1

Additional HSP90 Products

Product Documents for HSP90 Antibody (2D12)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP90 Antibody (2D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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