Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # OTI1H3

Concentration

1 mg/ml

View all HSPA8/HSC71 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSPA8(NP_006588) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

70.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSPA8/HSC71/Hsc70 Antibody (OTI1H3)

Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA8.
Immunocytochemistry/ Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746]

Immunocytochemistry/ Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746]

Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA8 .
Immunohistochemistry: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746]

Immunohistochemistry: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746]

Immunohistochemistry: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for HSPA8/HSC71/Hsc70 Antibody (OTI1H3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSPA8/HSC71

Hsp73 is a normal cell constituent which is synthesized in increased abundance under adverse environmental circumstances, such as non-physiological temperatures or treatment with amino acid analogues (1). Data has shown a molecular mechanism for cytokine-mediated posttranscriptional regulation of Bim mRNA by Hsc70, which binds to AU-rich elements (AREs) in the 3'-untranslated region of specific mRNAs and enhances their stability. The RNA binding potential of Hsc70 is regulated by cochaperones including Bag-4 (also SODD), CHIP, Hip, and Hsp40 (2). Hsp73 protein expression was detected in the cytoplasm of melanoma cells in primary tumors and metastases. In primary melanomas, the proportion of Hsp73 protein expressing cells correlated with tumor thickness which was found to be statistically significant (3).

Long Name

Heat Shock 70 kDa Protein 8

Alternate Names

HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, NIP71

Gene Symbol

HSPA8

Additional HSPA8/HSC71 Products

Product Documents for HSPA8/HSC71/Hsc70 Antibody (OTI1H3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HSPA8/HSC71/Hsc70 Antibody (OTI1H3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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