HSPB8/HSP22 Antibody (3C5)

Novus Biologicals, part of Bio-Techne | Catalog # H00026353-M12

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3C5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HSPB8 Antibodies »

Product Specifications

Immunogen

HSPB8 (AAH02673.1, 1 a.a. ~ 196 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADGQMPFSCHYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSSAWPGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLIIEAPQVPPYSTFGESSFNNELPQDSQEVTCT

Reactivity Notes

This product is reactive against Human.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for HSPB8/HSP22 Antibody (3C5)

Western Blot: HSPB8/HSP22 Antibody (3C5) [H00026353-M12]

Western Blot: HSPB8/HSP22 Antibody (3C5) [H00026353-M12]

Western Blot: HSPB8/HSP22 Antibody (3C5) [H00026353-M12] - Analysis of HSPB8 expression in HeLa(Cat # L013V1).
Sandwich ELISA: HSPB8/HSP22 Antibody (3C5) [H00026353-M12] - Detection limit for recombinant GST tagged HSPB8 is 0.03 ng/ml as a capture antibody.

Applications for HSPB8/HSP22 Antibody (3C5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is useful for ELISA, Western Blot

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSPB8

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Long Name

Heat Shock Protein beta 8

Alternate Names

CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22

Gene Symbol

HSPB8

UniProt

AAH02673 (Human)

Additional HSPB8 Products

Product Documents for HSPB8/HSP22 Antibody (3C5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HSPB8/HSP22 Antibody (3C5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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