HSPB8/HSP22 Antibody (5D7)
Novus Biologicals, part of Bio-Techne | Catalog # H00026353-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 5D7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HSPB8 (NP_055180, 97 a.a. ~ 196 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVCVNVHSFKPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLIIEAPQVPPYSTFGESSFNNELPQDSQEVTCT
Reactivity Notes
Human. Other species not tested.
Specificity
HSPB8 (5D7)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for HSPB8/HSP22 Antibody (5D7)
![Western Blot: HSPB8/HSP22 Antibody (5D7) [H00026353-M02]](https://resources.bio-techne.com/images/products/HSPB8-HSP22-Antibody-5D7-Western-Blot-H00026353-M02-img0004.jpg "Western Blot: HSPB8/HSP22 Antibody (5D7) [H00026353-M02]")
Western Blot: HSPB8/HSP22 Antibody (5D7) [H00026353-M02]
Western Blot: HSPB8/HSP22 Antibody (5D7) [H00026353-M02] - Analysis of HSPB8 expression in transfected 293T cell line by HSPB8 monoclonal antibody (M02), clone 5D7.Lane 1: HSPB8 transfected lysate(35.446 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: HSPB8/HSP22 Antibody (5D7) [H00026353-M02] - Detection limit for recombinant GST tagged HSPB8 is approximately 0.03ng/ml as a capture antibody.
Applications for HSPB8/HSP22 Antibody (5D7)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HSPB8
Long Name
Heat Shock Protein beta 8
Alternate Names
CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22
Entrez Gene IDs
26353 (Human)
Gene Symbol
HSPB8
OMIM
158590 (Human)
UniProt
Additional HSPB8 Products
Product Documents for HSPB8/HSP22 Antibody (5D7)
Product Specific Notices for HSPB8/HSP22 Antibody (5D7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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