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HSPC111 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89659

Novus Biologicals, part of Bio-Techne
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NBP1-89659
NBP1-89659-25ul

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for HSPC111 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LRKRKVKAMEVDIEERPKELVRKPYVLNDLEAEASLPEKKGNTLSRDLIDYVRYMVENHGEDYKAMAR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSPC111 Antibody

Western Blot: HSPC111 Antibody [NBP1-89659]

Western Blot: HSPC111 Antibody [NBP1-89659]

Western Blot: HSPC111 Antibody [NBP1-89659] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: HSPC111 Antibody [NBP1-89659]

Immunocytochemistry/ Immunofluorescence: HSPC111 Antibody [NBP1-89659]

Immunocytochemistry/Immunofluorescence: HSPC111 Antibody [NBP1-89659] - Staining of human cell line A-431 shows localization to nucleus, nucleoli & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HSPC111 Antibody [NBP1-89659]

Immunohistochemistry-Paraffin: HSPC111 Antibody [NBP1-89659]

Immunohistochemistry-Paraffin: HSPC111 Antibody [NBP1-89659] - Staining of human cerebellum shows strong nucleolar positivity in purkinje cells.

Applications for HSPC111 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSPC111

NOP16 is transcriptionally regulated by c-Myc (MYC; MIM 190080), upregulated in breast cancer, and overexpression is associated with poor patient survival (Butt et al., 2008).(supplied by OMIM)

Alternate Names

HBV pre-S2 trans-regulated protein 3, HSPC111HSPC185, LOC51491, NOP15, NOP16 nucleolar protein homolog (yeast), nucleolar protein 16, nucleolar protein 16 homolog, nucleolar protein 16 homolog (yeast)

Gene Symbol

NOP16

Additional HSPC111 Products

Product Documents for HSPC111 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSPC111 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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