HTRA2/Omi Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99409

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HTRA2/Omi Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human HTRA2/Omi (Accession#: O43464-1; Ala134-Glu458)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for HTRA2/Omi Antibody

Western Blot: HTRA2/Omi Antibody [NBP2-99409]

Western Blot: HTRA2/Omi Antibody [NBP2-99409]

Western Blot: HTRA2/Omi Antibody [NBP2-99409] - Anti-HTRA2/Omi rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: HL60 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 49 kDa. Observed band size: 37 kDa (We are unsure as to the identity of these extra bands).
Western Blot: HTRA2/Omi Antibody [NBP2-99409]

Western Blot: HTRA2/Omi Antibody [NBP2-99409]

Western Blot: HTRA2/Omi Antibody [NBP2-99409] - HTRA2/Omi was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg HL-60 Whole Cell Lysate. 1 ul anti-HTRA2/Omi rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-HTRA2/Omi rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 37 kDa

Applications for HTRA2/Omi Antibody

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HTRA2/Omi

HtrA2 (Omi) is a serine protease that plays a significant role in programmed cell death, or apoptosis. Localized to the intermembrane space of the mitochondria, HtrA2 exists predominantly as two isoforms (1). In response to cellular stress signals, HtrA2 is released into the cytosol. One way that HtrA2 induces apoptosis is by binding to BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), and thereby inhibiting their caspase-inhibitory activity (2,3).

Long Name

High Temperature Requirement Protein-2

Alternate Names

Omi, PRSS25

Gene Symbol

HTRA2

Additional HTRA2/Omi Products

Product Documents for HTRA2/Omi Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HTRA2/Omi Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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