Human 11 beta-HSD2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8630
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 921215
Product Specifications
Immunogen
E. coli-derived recombinant human 11 beta‑HSD2
Met105-Arg405
Accession # P80365
Met105-Arg405
Accession # P80365
Specificity
Detects human 11 beta‑HSD2 in direct ELISA and Western Blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human 11 beta-HSD2 Antibody
Detection of Human 11 beta-HSD2 by Western Blot.
Western blot shows lysates of human kidney tissue, human placenta tissue, and human colon tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human 11 beta-HSD2 Monoclonal Antibody (Catalog # MAB8630) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 11 beta-HSD2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
11 beta‑HSD2 in Human Kidney.
11 beta-HSD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human 11 beta-HSD2 Monoclonal Antibody (Catalog # MAB8630) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human 11 beta-HSD2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney
Sample: Immersion fixed paraffin-embedded sections of human kidney
Western Blot
1 µg/mL
Sample: Human kidney tissue, human placenta tissue, and human colon tissue
Sample: Human kidney tissue, human placenta tissue, and human colon tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 11 beta-HSD2
Long Name
11 beta Hydroxysteroid Dehydrogenase 2
Alternate Names
11 betaHSD2, HSD11B2
Gene Symbol
HSD11B2
UniProt
Additional 11 beta-HSD2 Products
Product Documents for Human 11 beta-HSD2 Antibody
Product Specific Notices for Human 11 beta-HSD2 Antibody
For research use only
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