Human 17 beta-HSD1/HSD17B1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7178
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 860020
Product Specifications
Immunogen
E. coli-derived recombinant human 17 beta‑HSD1/HSD17B1
Ala2-Gln328
Accession # P14061
Ala2-Gln328
Accession # P14061
Specificity
Detects human 17 beta‑HSD1/HSD17B1 in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human 17 beta-HSD1/HSD17B1 Antibody
Detection of Human 17 beta‑HSD1/ HSD17B1 by Western Blot.
Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human 17 beta-HSD1/HSD17B1 Monoclonal Antibody (Catalog # MAB7178) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for 17 beta-HSD1/HSD17B1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human 17 beta‑HSD1/HSD17B1 by Simple WesternTM.
Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for 17 beta‑HSD1/HSD17B1 at approximately 40 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human 17 beta‑HSD1/HSD17B1 Monoclonal Antibody (Catalog # MAB7178). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Applications for Human 17 beta-HSD1/HSD17B1 Antibody
Application
Recommended Usage
Simple Western
2.5 µg/mL
Sample: Human placenta tissue
Sample: Human placenta tissue
Western Blot
0.25 µg/mL
Sample: Human placenta tissue
Sample: Human placenta tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB7178 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 17 beta-HSD1/HSD17B1
Long Name
17 beta Hydroxysteroid Dehydrogenase 1
Alternate Names
17 betaHSD1, E2DH, EDH17B2, EDHB17, HSD17B1, SDR28C1
Gene Symbol
HSD17B1
UniProt
Additional 17 beta-HSD1/HSD17B1 Products
Product Documents for Human 17 beta-HSD1/HSD17B1 Antibody
Product Specific Notices for Human 17 beta-HSD1/HSD17B1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...