Human 20S Immunoproteasome Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB81941
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 894505
Product Specifications
Immunogen
Human 20S Immunoproteasome purified from peripheral blood mononuclear cells
Specificity
Detects Human 20S Immunoproteasome in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human 20S Immunoproteasome Antibody
20S Immunoproteasome in Human Lung Cancer Tissue.
20S Immunoproteasome was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human 20S Immunoproteasome Polyclonal Antibody (Catalog # MAB81941) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cell cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human 20S Immunoproteasome Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human lung cancer tissue
Reviewed Applications
Read 1 review rated 5 using MAB81941 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 20S Immunoproteasome
The 20S Immunoproteasome is a modified form of the constitutively active 20S Proteasome core particle and is the catalytic subunit of the multi-complex Immunoproteasome. The structure of the 20S Immunoproteasome is similar to the 20S Proteasome, which is composed of 28 non-identical subunits arranged into four stacked rings. However, during 20S Immunoproteasome assembly, the three catalytic beta subunits, beta 1, 2, and 5, in the two interior rings of the 20S Proteasome are replaced by three IFN-gamma-inducible catalytic subunits: beta 1i/LMP2, beta 2i/LMP7, and beta 5i/MECL-1. The 20S Immunoproteasome is commonly associated with the 19S, PA28 alpha/beta, or the PA28 gamma regulatory complexes. 20S Immunoproteasome expression is enriched in antigen presenting cells of the immune system where the 20S Immunoproteasome selectively degrades intracellular proteins in a manner that optimizes the generation of peptides for MHC class I antigen presentation.
Additional 20S Immunoproteasome Products
Product Documents for Human 20S Immunoproteasome Antibody
Product Specific Notices for Human 20S Immunoproteasome Antibody
For research use only
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