Human 4-1BB Ligand/TNFSF9 Alexa Fluor 350 Antibody (282220)
R&D Systems, part of Bio-Techne | Catalog # FAB2295U
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 282220
Product Specifications
Immunogen
E. coli-derived recombinant human 4‑1BB Ligand/TNFSF9
Arg71-Glu254
Accession # P41273.1
Arg71-Glu254
Accession # P41273.1
Specificity
Detects human 4‑1BB Ligand/TNFSF9 Ligand in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) 4-1BB Ligand, recobinant human (rh) APRIL, rhBAFF, rmEDA-A2, rhFas Ligand, rhGITR Ligand, rhLIGHT, rhLymphotoxin alpha1/ beta2, rhLymphotoxin alpha2/ beta1, rhOX40 Ligand, rhTNF-alpha, rhTRAIL, rhTRANCE, or rhTWEAK is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human 4-1BB Ligand/TNFSF9 Alexa Fluor 350 Antibody (282220)
Application
Recommended Usage
Flow Cytometry
0.25-1 µg/106 cells
Sample: Daudi human Burkitt's lymphoma cell line
Sample: Daudi human Burkitt's lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: 4-1BB Ligand/TNFSF9
4-1BB Ligand (4-1BBL; also CD137L) is a 32 kDa type II transmembrane protein that belongs to the TNF superfamily (TNFSF) molecules (1‑4). The human 4-1BBL cDNA encodes a 254 amino acid (aa) protein that contains a 25 aa N-terminal cytoplasmic domain, a 23 aa transmembrane segment, and a 206 aa C-terminal extracellular region (5). The extracellular domain (ECD) of 4-1BBL has a jelly-roll, beta-sandwich tertiary structure that is similar to other TNFSF members. There is only one cysteine in the human ECD, and no potential N-linked glycosylation sites. The potential exists, however, for O-linked glycosylation. The human 4-1BBL ECD shares 32% and 35% aa identity with mouse and rat ECD, respectively. In the cytoplasmic domain, human 4-1BBL is 55 aa shorter than the equivalent region in rodents. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes (2, 3, 6). A bioactive 26 kDa soluble form of 4-1BBL, presumably generated by MMP cleavage, occurs in humans (4). Human 4-1BBL signals through both CD137/4-1BB and itself. Its cytoplasmic tail participates in reverse signaling that induces apoptosis in T cells and cytokine secretion (IL-6; TNF-alpha) by monocytes (7, 8). 4-1BBL binding to CD137/4-1BB produces a number of effects. It seems to play a key role in the T cell recall response. It maintains T cell numbers at the end of a primary response, and induces CD4+ and CD8+ T cells to proliferate and secrete cytokines such as IL-2 and IFN-gamma in CD4+ cells, and IFN-gamma in CD8+ cells (9, 10).
References
- Kwon, B. et al. (2003) Exp. Mol. Med. 35:8.
- Salih, H.R. et al. (2002) Int. J. Clin. Pharmacol. Ther. 40:348.
- Vinay, D.S. and B.S. Kwon (1998) Semin. Immunol. 1:481.
- Salih, H.R. et al. (2001) J. Immunol. 167:4059.
- Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219.
- Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
- Michel, J. et al. (1999) Immunology 98:42.
- Langstein, J. et al. (1998) J. Immunol. 160:2488.
- Wen, T. et al. (2002) J. Immunol. 168:4897.
- Dawicki, W. and T.H. Watts (2004) Eur. J. Immunol. 34:743.
Alternate Names
41BB Ligand, CD137L, TNFSF9
Gene Symbol
TNFSF9
UniProt
Additional 4-1BB Ligand/TNFSF9 Products
Product Documents for Human 4-1BB Ligand/TNFSF9 Alexa Fluor 350 Antibody (282220)
Product Specific Notices for Human 4-1BB Ligand/TNFSF9 Alexa Fluor 350 Antibody (282220)
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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