Human 5T4 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF4975
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human 5T4
Ser31-Ser355
Accession # Q13641
Ser31-Ser355
Accession # Q13641
Specificity
Detects human 5T4 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse 5T4 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human 5T4 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human 5T4
Sample: Recombinant Human 5T4
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: 5T4
Human 5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium, and embryonic stem cells and impacts cell adhesion and motility. The human 5T4 cDNA encodes a type I transmembrane protein precursor that is 420 amino acids (aa) in length. It contains a 324 aa extracellular region (aa 32-355) that shows one Ser-rich region followed by seven Leu-rich repeats (aa 90-355). Over aa 31-355, human 5T4 shares 81% and 85% aa identity with mouse and canine 5T4, respectively.
Long Name
Oncofetal Antigen/Trophoblast Glycoprotein
Alternate Names
M6P1, TPBG
Gene Symbol
TPBG
UniProt
Additional 5T4 Products
Product Documents for Human 5T4 Biotinylated Antibody
Product Specific Notices for Human 5T4 Biotinylated Antibody
For research use only
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