Human A20/TNFAIP3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB75981
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 775928
Product Specifications
Immunogen
E. coli-derived recombinant human A20/TNFAIP3
Lys91-Leu263
Accession # P21580
Lys91-Leu263
Accession # P21580
Specificity
Detects human A20/TNFAIP3 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human A20/TNFAIP3 Antibody
A20/TNFAIP3 in HL‑60 Human Cell Line.
A20/TNFAIP3 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB75981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of A20/TNFAIP3 in Normal Breast.
A20/TNFAIP3 was detected in immersion fixed paraffin-embedded sections of normal breast using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB75981) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and glandular cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of A20/TNFAIP3 in Normal Liver.
A20/TNFAIP3 was detected in immersion fixed paraffin-embedded sections of normal liver using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB75981) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human A20/TNFAIP3 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HL‑60 human acute promyelocytic leukemia cell line
Sample: Immersion fixed HL‑60 human acute promyelocytic leukemia cell line
Immunohistochemistry
3-25 µg/mL
Sample:
Sample:
Immersion fixed paraffin-embedded sections of normal breast and normal liver
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: A20/TNFAIP3
Alternate Names
OTUD7C, TNFA1P2, TNFAIP3
Gene Symbol
TNFAIP3
UniProt
Additional A20/TNFAIP3 Products
Product Documents for Human A20/TNFAIP3 Antibody
Product Specific Notices for Human A20/TNFAIP3 Antibody
For research use only
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