Human A33 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB3080A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 402104
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795
Ile22-Val235
Accession # Q99795
Specificity
Detects human A33 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse A33 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human A33 APC-conjugated Antibody
Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry.
HT-29 human colon adenocarcinoma cell line was stained with Rat Anti-Human A33 APC-conjugated Monoclonal Antibody (Catalog # FAB3080A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of A33 in Colo205 cells by Flow Cytometry.
Colo205 cells were stained with Rat Anti-Human A33 APC-conjugated Monoclonal Antibody (Catalog # FAB3080A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human A33 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: HT‑29 human colon adenocarcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line.
Sample: HT‑29 human colon adenocarcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: A33
Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.
Long Name
Cell Surface A33 Antigen
Alternate Names
GPA33
Gene Symbol
GPA33
UniProt
Additional A33 Products
Product Documents for Human A33 APC-conjugated Antibody
Product Specific Notices for Human A33 APC-conjugated Antibody
For research use only
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