Human ABCG2 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM995
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry
Cited:
Immunocytochemistry
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2B Clone # 5D3
Product Specifications
Immunogen
3T3 cells transduced with human ABCG2
Specificity
Detects human ABCG2.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human ABCG2 Biotinylated Antibody
ABCG2 in A549 Human Cell Line.
ABCG2 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human ABCG2 Biotinylated Monoclonal Antibody (Catalog # BAM995) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human ABCG2 Biotinylated Antibody
Application
Recommended Usage
Flow Cytometry
2.5 µg/106 cells
Sample: Human MCF-7 cell line
Sample: Human MCF-7 cell line
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line
Sample: Immersion fixed A549 human lung carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ABCG2
References
- Chaudhary, P.M. and I.B. Roninson (1991) Cell 66:85.
- Sorrentino, B.P. et al. (1995) Blood 86:491.
- Pallis, M. and N. Russell (2000) Blood 95:2897.
- Johnstone, R.W. et al. (1999) Blood 93:1075.
- Doyle, L.A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15665.
- Zhou, S. et al. (2001) Nat. Medicine 7:1028.
- Hrycyna, C.A. et al. (1998) Biochem. 37:13660.
- Bunting, K.D. (2002) Stem Cells 20:11.
Long Name
ATP-binding Cassette Transporter G2
Alternate Names
ABCP, BCRP, CD338, MXR
Entrez Gene IDs
9429 (Human)
Gene Symbol
ABCG2
Additional ABCG2 Products
Product Documents for Human ABCG2 Biotinylated Antibody
Product Specific Notices for Human ABCG2 Biotinylated Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...