Detects the ectodomain of recombinant human (rh) ADAM15 in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross-reactivity with recombinant mouse (rm) ADAM15 but does not cross-react with rhADAM8, 9, 17, 28, rmADAM9, or 10.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human ADAM15 Ectodomain Antibody

ADAM15 in MCF‑7 Human Cell Line.

ADAM15 was detected in immersion fixed MCF-7 human breast cancer cell line using Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

ADAM15 in Human Prostate Cancer Tissue.

ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of ADAM15 in PC-3 cells (Positive) & MOLT-4 cells (Negative).

ADAM15 was detected in immersion fixed PC-3 human prostate cancer cells (Positive) & absent in MOLT-4 human acute lymphoblastic leukemia cells (Negative) using Mouse Anti-Human ADAM15 Ectodomain Monoclonal Antibody (Catalog # MAB935) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Applications for Human ADAM15 Ectodomain Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line, PC-3 human prostate cancer cells (Positive) & absent in MOLT-4 human acute lymphoblastic leukemia cells (Negative)

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate cancer tissue

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAM15, see our available Western blot detection antibodies

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: MCF-7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)
under non-reducing conditions only

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB935 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM15

ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Long Name

A Disintegrin and Metalloprotease-like Domain 15

Alternate Names

MDC15, Metargidin

Entrez Gene IDs

8751 (Human); 11490 (Mouse)

Gene Symbol

ADAM15

UniProt

Q13444

Additional ADAM15 Products

Product Documents for Human ADAM15 Ectodomain Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ADAM15 Ectodomain Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ADAM15 Ectodomain Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB935

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications