Human ADAMTS3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5465

R&D Systems, part of Bio-Techne
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AF5465
AF5465-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all ADAMTS3 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS3
Ser19-Cys712
Accession # O15072

Specificity

Detects human ADAMTS3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 3% cross-reactivity with recombinant human (rh) ADAMTS4 and less than 1% cross-reactivity with rhADAMTS1, rhADAMTS5, rhADAMTS13, and rhADAMTSL1.2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human ADAMTS3 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS3, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human ADAMTS3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS3

ADAMTS3 (A disintegrin and metalloprotease with thrombospondin motifs 3; also PC II-NP) is a 140-150 kDa member of the ADAMTS family of Zn metalloproteases. It is expressed by osteoblasts, chrondrocytes, myoepithelium and fibroblasts, and participates in collagen maturation. Together with ADAMTS2 and TS14, ADAMTS3 is known to process the N-terminus of procollagen. In particular, it cleaves the N-terminal globular region of collagen I and II, leading to fibril formation. Mature human proADAMTS3 is a secreted, 1185 amino acid (aa) glycoprotein. It is highly modular and contains a proregion (aa 38-201), a peptidase M12B domain (aa 256-460), a disintegrin region (aa 470-550), four TSP type I sequences (aa 551-1015), and a PLAC domain (aa 1015-1054). There are two potential splice variants. One shows a 38 aa substitution for aa 169-1205, while another shows an alternative start site at Met171. Over aa 19-712, human ADAMTS3 shares 91% aa identity with mouse ADAMTS3.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 3

Alternate Names

PC II-NP

Entrez Gene IDs

9508 (Human); 330119 (Mouse); 305253 (Rat)

Gene Symbol

ADAMTS3

UniProt

O15072 (Human)

Additional ADAMTS3 Products

Product Documents for Human ADAMTS3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ADAMTS3 Antibody

For research use only

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