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Human ADAMTS4 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4307

R&D Systems, part of Bio-Techne
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AF4307
AF4307-SP

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS4
Phe213-Cys685
Accession # O75173

Specificity

Detects human ADAMTS4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) ADAMTS5 is observed and less than 1% cross-reactivity with rhADAMTS13, rhADAMTS-L1.2, and rhADAMTS1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human ADAMTS4 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ADAMTS4 (Catalog # 4307‑AD), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human ADAMTS4 (Catalog # 4307-AD)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAMTS4

ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs 4), also known as aggrecanase-1, is a member of the family of secreted zinc proteases with a multi-domain structure (1‑3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha-2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4‑6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). The purified rhADAMTS4 starts at the catalytic domain and ends before the spacer domain. If desired, the aggrecanase activity can be inhibited by 5 mM 1,10‑phenanthroline and recombinant human TIMP-3 (R&D Systems, Catalog # 973-TM).

References

  1. Totorella, M. D. et al. (1999) Science 284:1664.
  2. Porter, S. et al. (2005) Biochem. J. 386:15.
  3. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
  4. Tortorella, M. D. et al. (2004) J. Biol. Chem. 279:17554.
  5. Struglics, A. et al. (2006) Osteoarthritis Cartilage. 14:101.
  6. Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501.
  7. Pratta, M. A. et al. (2003) Arthritis Reum. 48:119.
  8. Glasson, S. S. et al. (2004) Arthritis Reum. 50:2547.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 4

Alternate Names

ADMP-1, Aggrecanase 1

Entrez Gene IDs

9507 (Human); 66015 (Rat)

Gene Symbol

ADAMTS4

UniProt

Additional ADAMTS4 Products

Product Documents for Human ADAMTS4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human ADAMTS4 Antibody

For research use only

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