HEK293 human embryonic kidney cell line transfected with either (A) human AdipoR1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human AdipoR1 Monoclonal Antibody (Catalog # MAB10128) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

AdipoR1 in Human Placenta.

AdipoR1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human AdipoR1 Monoclonal Antibody (Catalog # MAB10128) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in decidual cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human AdipoR1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human AdipoR1 and eGFP

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AdipoR1

Adiponectin receptor protein 1 (AdipoR1) is a 7 transmembrane receptor for globular and full-length adiponectin required for normal glucose and fat homeostasis and for maintaining a normal body weight. AdipoR1 mediates increased AMP kinase and PPAR-alpha ligand activities well as fatty-acid oxidation and glucose uptake by adiponectin. Genetic variability at the AdipoR1 locus is a strong determinant of coronary artery disease susceptibility in type 2 diabetes. AdipoR1 is abundantly expressed in skeletal muscle.

Long Name

Adiponectin Receptor 1

Alternate Names

ACDCR1, CGI45, PAQR1, TESBP1A

Entrez Gene IDs

51094 (Human); 72674 (Mouse); 289036 (Rat)

Gene Symbol

ADIPOR1

Additional AdipoR1 Products

Product Documents for Human AdipoR1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human AdipoR1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human AdipoR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10128

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all AdipoR1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human AdipoR1 Antibody

Detection of AdipoR1 in HEK293 Human Cell Line Transfected with Human AdipoR1 and eGFP by Flow Cytometry.