Human Aggrecan (ADAMTS5-cleaved) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6489
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Clone # 661725
Product Specifications
Immunogen
KLHG-conjugated peptide from human Aggrecan, Leu384-Glu392
Accession # P16112
Accession # P16112
Specificity
Detects human Aggrecan in direct ELISAs. It specifically recognizes the N-terminal fragment of recombinant human Aggrecan released following cleavage by ADAMTS5.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3
Applications for Human Aggrecan (ADAMTS5-cleaved) Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample:
Sample:
Conditioned cell culture medium spiked with ADAMTS5 cleaved recombinant human aggrecan, see our available Western blot detection antibodies
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aggrecan
Aggrecan, together with type II collagen, makes up to 90% of the dry weight of healthy cartilage. It hydrates the collagen network and thus provides cartilage with its properties of compressibility and elasticity. The N-terminal region of human Aggrecan (residues 20‑675) consists of two globular domains (G1 and G2), flanking an interglobular domain (IGD). The IGD contains the cleavage sites for matrix metalloproteinases and aggrecanases.
Alternate Names
ACAN, AGC1, CSPG1, CSPGCP, MSK16, SEDK
Entrez Gene IDs
176 (Human)
Gene Symbol
ACAN
UniProt
Additional Aggrecan Products
Product Documents for Human Aggrecan (ADAMTS5-cleaved) Antibody
Product Specific Notices for Human Aggrecan (ADAMTS5-cleaved) Antibody
For research use only
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