Human AHR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB61851
Recombinant Monoclonal Antibody.
Discontinued Product
MAB61851 has been discontinued.
View all AHR products.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1167A
Product Specifications
Immunogen
E. coli-derived recombinant human AHR
Asn704-Leu848
Accession # P35869
Asn704-Leu848
Accession # P35869
Specificity
Detects human AHR in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human AHR Antibody
Detection of Human AHR by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human AHR Monoclonal Antibody (Catalog # MAB61851) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for AHR at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
AHR in HeLa Human Cell Line.
AHR was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human AHR Monoclonal Antibody (Catalog # MAB61851) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human AHR Antibody
Application
Recommended Usage
Immunocytochemistry
1-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AHR
AHR (Aryl-hydrocarbon receptor; also known as bHLHE76) is a 110 kDa member of the bHLH/PAS transcription factor family. It is widely expressed (breast, lung, liver), and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a down‑regulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing TP73 and Apaf1 genes. Human AHR is 848 amino acids (aa) in length. It contains a 10 aa prosegment, plus a 838 aa mature molecule that contains a DNA binding motif (aa 13-40), a bHLH region (aa 41-81), and two PAS domains (aa 111-342). Over aa 704-848, human AHR shares 70% aa identity with mouse AHR.
Long Name
Aryl Hydrocarbon Receptor
Alternate Names
BHLHE76
Gene Symbol
AHR
UniProt
Additional AHR Products
Product Documents for Human AHR Antibody
Product Specific Notices for Human AHR Antibody
For research use only
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