Rabbit IgG Horseradish Peroxidase-conjugated Antibody Best Seller
R&D Systems, part of Bio-Techne | Catalog # HAF008
Key Product Details
Validated by
Biological Validation
Species Reactivity
Rabbit
Applications
Immunohistochemistry, Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Rabbit IgG
Specificity
Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rabbit IgG Horseradish Peroxidase-conjugated Antibody
Detection of Rabbit IgG Primary Antibody by Western Blot
Western blot shows detection of Rabbit Anti-Human/Mouse/Rat HSP60 Polyclonal Antibody (AF1800) using a 1:1000 dilution of HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of FoxP3 in Human Tonsil
Detection of FoxP3 in human tonsil using 5 μg/mL of Rabbit monoclonal anti-human FoxP3 primary antibody (MAB8214) followed by 30 min incubation with Goat anti Rabbit HRP conjugated secondary antibodies (Catalog # HAF008) at 1:20 dilution + DAB chromogen (brown). The tissue was counterstained with Hematoxylin (blue). Control was done by omitting primary antibody.
Detection of Human IgG by Western Blot
PARP-cleavage upon treatment with embryonic stimuli.Representative Western Blot analysis of PARP cleavage (full length PARP 116kDa; cleaved PARP 89 kDa) in non-differentiated (St-T1, lanes 1+2; KdS1, lanes 3+4) and decidualized (dSt-T1, lanes 5+6; dKdS1, lanes 7+8) ESCs after treatment with IITT+F vs. untreated controls, n = 3; beta-Actin (42kDa) served as a loading control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0121103), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Rabbit IgG Horseradish Peroxidase-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Use at a 1:20 dilution
Western Blot
Use at a 1:1000 dilution.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 5 reviews rated 5 using HAF008 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Rabbit IgG Horseradish Peroxidase-conjugated Antibody
Product Specific Notices for Rabbit IgG Horseradish Peroxidase-conjugated Antibody
For research use only
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