Human AICL/CLEC-2B Antibody

Detection of Human AICL/CLEC-2B by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human AICL/CLEC-2B Monoclonal Antibody (Catalog # MAB90591) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AICL/CLEC-2B at approximately 24-26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of AICL/CLEC-2B in U937 cells (positive), T-47 cells (negative) and A431 cells (negative).

AICL/CLEC-2B was detected in 10% Formalin fixed U937 human histiocytic lymphoma cell line (Positive), absent in T47D human breast cancer cell line (Negative) and absent in A431 human epithelial carcinoma cell line (Negative) using Mouse Anti-Human AICL/CLEC-2B Monoclonal Antibody (Catalog # MAB90591) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of AICL/CLEC-2B in Human Tonsil.

AICL/CLEC-2B was detected in Formalin fixed paraffin-embedded sections of Human Tonsil using Mouse Anti-Human AICL/CLEC-2B Monoclonal Antibody (Catalog # MAB90591) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with Hematoxylin (blue). Specific staining was localized to Cytoplasmic. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.