Human AIF-1/Iba1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7308
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry
Cited:
Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 603102
Product Specifications
Immunogen
E. coli-derived recombinant human AIF-1/Iba1
Met1-Pro147
Accession # P55008
Met1-Pro147
Accession # P55008
Specificity
Detects human AIF-1/Iba1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human AIF-1/Iba1 Antibody
AIF-1/Iba1 in Human Spleen.
AIF-1/Iba1 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human AIF-1/Iba1 Monoclonal Antibody (Catalog # MAB7308) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human AIF-1/Iba1 Antibody
Application
Recommended Usage
Immunohistochemistry
0.5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen
Sample: Immersion fixed paraffin-embedded sections of human spleen
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB7308 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AIF-1/Iba1
aa 1-54 as well as aa 121-147 but also has a 66 aa insertion at position 65.
Long Name
Allograft Inflammatory Factor 1
Alternate Names
AIF1, IBA1, IRT1
Gene Symbol
AIF1
UniProt
Additional AIF-1/Iba1 Products
Product Documents for Human AIF-1/Iba1 Antibody
Product Specific Notices for Human AIF-1/Iba1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...